Coding

Part:BBa_K1152010:Design

Designed by: Ralf Beer, Konrad Herbst, Nikolaos Ignatiadis   Group: iGEM13_Heidelberg   (2013-09-19)

Svp 4'-Phosphopanthetheinyl-transferase

  • CDS of svp derived from STreptomyces verticillus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 173
    Illegal NgoMIV site found at 413
    Illegal NgoMIV site found at 663
    Illegal AgeI site found at 573
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 366


Design Notes

The svp CDS is derived directly form genomic DNA of S. verticillus ATCC15003. [Takahashi, 1997] We cloned sfp in a pSB3K3-derived plasmid with a lac promoter (BBa_R0010) and the RBS BBa_B0029.


Source

We amplified svp from Streptomyces verticillus ATCC15003 [Takahashi, 1997]

References

  • Takahashi, H., Kumagai, T., Kitani, K., Mori, M., Matoba, Y., & Sugiyama, M. (2007). Cloning and Characterization of a Streptomyces Single Module Type Non-ribosomal Peptide Synthetase Catalyzing a Blue Pigment Synthesis *, 282(12), 9073–9081. doi:10.1074/jbc.M611319200